Environ. Horticulture, Pears, Cherries, and Viticulture
University of California
Environ. Horticulture, Pears, Cherries, and Viticulture

Posts Tagged: Drosophila suzukii

Length and Magnitude of Fruit Cooling and Spotted Wing Drosophila Mortality

In some of the first literature written in Japan in 1939 (Kanzawa, T.) about spotted wing drosophila, Drosophila suzukii, (SWD), experiments were made regarding the sensitivity of the egg and larval stages of spotted wing drosophila to periods of temperatures above and below freezing (32o F).

As is noted in the two graphs below, at constant temperatures of up to 35o F, 96 hours or more of cooling resulted in total mortality of spotted wing drosophila eggs and larvae.  This was also anecdotally confirmed in tests conducted in 2009 in California.

While temperatures below freezing are not useful to fruit shippers, temperatures in the area of 35o F are.  However, it is important to note that for success the constancy of the temperature is critical.  So, while in an ideal situation constant temperatures of 35o F or a little below are effective in SWD egg and larvae suppression when extended for periods longer than 96 hours, the reality can vary significantly from the ideal.  Shipped fruit ordinarily do not experience lengthy regimes of constant temperature as they are moved from place to place.  Temperatures of a refrigerator truck can vary by location inside and placement of the produce (ie on the side, towards the bottom etc.), and certainly the temperatures at the point of sale can vary from the ideal to room temperature to even warmer.  

Additionally, while initial damage from SWD on raspberries, blackberries and strawberries can be difficult to detect, this is not the case for other fruits such as cherries or blueberries, where the activity of SWD will leave an unsightly blemish.

The take home message from this information is that while extended cooling can be suppressive of SWD, growers should not rely on cooling alone.  It will still be important to manage SWD in field.

Thanks to Shinji Kawai for making the information from the 1939 Kanzawa paper available.

Graph courtesy of Ed Show
Graph courtesy of Ed Show

Bar graph demonstrating higher mortality of SWD eggs beyond 96 hours at temperatures above and below freezing.

Graph courtesy of Ed Show
Graph courtesy of Ed Show

Bar graph demonstrating higher mortality of SWD larvae beyond 96 hours at temperatures above and below freezing.

Posted on Tuesday, March 23, 2010 at 4:39 PM

Further Chemical Efficacy Trials for Management of Spotted Wing Drosophila, Drosophila suzukii, in Raspberries.

Introduction:  An August 2009 field trial demonstrated the efficacy of  malathion and zeta-cypermethrin (Mustang) against the spotted winged drosophila (SWD), Drosophila suzukii in raspberries.  The following summary reports efficacy of additional materials trialed against SWD in December 2009.

Materials and Methods:

Treatments:

Chemical

Treatment Rate per acre

Water Carrier Rate per acre

Malathion

64 fl oz  

179 gallons

Delegate (spinetoram)

6 oz

179 gallons

HGW86 (Cyantraniliprole) Not Registered in Caneberry

398.6 ml

179 gallons

Altacor (Chlorantraniliprole)
Not Registered in Caneberry

64 fl oz

179 gallons

Actara (Thiamethoxam)

129.6 g

179 gallons

Untreated Control

-

-

Products were applied on December 3, 2009 with a Maruyama 056 gas powered back pack sprayer at maximum labeled rates in 179 gallons water per acre and 150 psi.  Each plot was 1,173 sq ft of a proprietary raspberry variety under polyethylene-covered tunnels.

Pre- and post-treatment estimations of SWD were made with a D-Vac Model 122 (hand carry) gas-powered insect sampling device operated at an airflow capacity of 280 cu ft per minute per square foot at the opening of the collecting head.  Each side of a 36-ft section of 4-ft high hedge row was sampled.  Collected samples were transferred from the D-Vac into Ziploc bags for transport back to the lab where they were frozen for approximately an hour prior to counting.

Because male SWD (easily distinguished by the black spot on their forewings) comprised over half the total Drosophila captured in most samples, we assumed the remaining fraction of Drosophila to be female SWD (which do not have spotted wings).  However, since the species of the females was not confirmed, they will be given in results as "females". Results are reported as total (male + female) SWD.

In order to evaluate the impact of treatments on incidence of fruit infestation by larval SWD, thirty marketable fruit were collected from the center of each treatment replicate prior to treatment and then 7-, 14-, 21- and 28-days post-application.  Fruit samples were placed in 4x6x12-inch plastic bags fixed with a 1x ¾-inch PVC screened vent tube sealing the open ended of the rearing bag (see photo below).  After two weeks, the numbers of larvae, pupae and adults were enumerated.

Results:

Table 1: Total numbers of male + "female" SWD captured in D-Vac samples

 

Pre-application

Post One Day

Post One Week

Post Two Weeks

Post Three Weeks

 

12/3/2009

12/4/2009

12/9/2009

12/16/2009

12/23/2009

Altacor

8.0 a

28.7 a

3.3 bc

49.3 a

6.3 a

Actara

13.0 a

38.0 a

 6.0 abc

48.7 a

6.0 a

UTC

17.7 a

38.0 a

7.3 ab

62.3 a

4.7 a

Delegate

8.3 a

18.0 a

2.3 bc

18.7 a

4.7 a

Malathion

12.7 a

10.3 a

1.7 c

14.3  a

1.0 a

HGW86

12.0 a

33.0 a

9.0 a

46.7 a

2.3 a

Means followed by the same letter do not significantly differ (P=0.05, Student-Newman Keuls)

Table 2: Infested fruit

 

Pre-Application

Post 1Week

Post 2 Weeks

Post 3 Weeks

 

12/3/2009

12/9/2009

12/16/2009

12/23/2009

Altacor

10.3 a

2.7 b

0.3 b

4.3 ab

Actara

4.7 ab

 3.0 b

1.7 b

2.3 ab

UTC

8.7 ab

9.0 a

0.7 b

6.3 a

Delegate

3.3 b

0.3 b

0.0 b

0.0 b

Malathion

8.7 ab

2.0 b

0.7 b

0.3 b

HGW86

6.0 ab

1.7 b

0.7 b

3.3 ab

Means followed by the same letter do not significantly differ (P=0.05, Student-Newman Keuls)

The effect of Delegate and Malathion was most notable in the evaluation of infested fruit (Table 2).  Although all treated fruit collected one week after application demonstrated significantly lower levels of infestation than the untreated control, Delegate and Malathion treatments continued to show significant levels of control up to three weeks after application.

Conclusion: This study demonstrates that Delegate is as effective as malathion in controlling SWD at relatively low population levels as those encountered during December 2009.

There are several insecticides mentioned for control of vinegar flies in this article.  Before using any insecticides, check with your local Agricultural Commissioner's Office and consult product labels for current status of product registration, restrictions, and use information.

We are very grateful for the cooperation of Dutra Farms and Chris Hogan for providing the test site.

Fruit infestation study
Fruit infestation study

30 raspberry fruit are collected into each plastic bag to incubate out SWD. Note the vented tube at the top of each bag to provide air exchange. Green sticks are to keep bags upright.

Posted on Thursday, January 21, 2010 at 1:37 PM

Update on the Cherry Vinegar Fly, Drosophila suzukii , Now Known as the Spotted Wing Drosophila

This a brief summary of the information presented at yesterday’s meeting in Stockton about the cherry vinegar fly, now known as the spotted wing drosophila (SWD), Drosophila suzukii. The purpose of the meeting was to update researchers, growers and industry personnel on the status of this pest.
 
Spotted wing drosophila has now been found in cherries, raspberries, blackberries, blueberries, and strawberries in California. It has been found in many (I think the number was 21) counties across California, as well as several other states. However, in the case of detections from other states, it has not yet been determined whether the detections come from fruit grown in those states or simply were fruit from infested areas of California incidentally brought back into California. 
 
Of the 3000 species of Drosophila commonly known as vinegar flies only two have been found to be harmful to crops, of which the spotted wing drosophila is one.   The female has a serrated ovipositor, meaning she can penetrate the skin of most thin skinned fruit. In the lab it takes from 2-3 minutes to oviposit, and this oviposition is not necessarily successful each time. Nevertheless, in an experiment done by Artyom Kopp from UC Davis, three females put in a test tube with a Thompson seedless grape had several dozen eggs laid overnight. A female will lay 2-3 eggs per fruit, and will lay around 350 eggs in her lifetime. The life cycle in the laboratory, from egg to egg laying female, is 12 to 15 days at 65o F. The egg and larvae develop inside of the fruit, and while experts at the meeting said that pupation takes place inside of the fruit also, I have taken many samples of strawberry and raspberry and they are always exiting to pupate.
 
It is thought that SWD will have ten generations per year, and probably 3 generations in one crop of cherries. Thus, the population growth potential of this pest is tremendous. They are most active at 68o F and activity becomes reduced at temperatures above 86o F. Above 86o F, males become sterile. Eggs, larvae and adults (probably pupae are not far behind) die at temperatures below freezing, but this does not necessarily mean a whole population will be eradicated at lower temperatures, since D. suzukii is firmly established on the island of Hokkaido in Japan, where winters are quite cold. Additionally, vinegar flies in general are very sensitive to dessication, and will die within 24 hours in the absence of water.
 
On the regulatory side, we were informed that neither the CDFA nor the USDA are recommending any regulatory action (such as a quarantine) at this time. On a conference call regarding SWD with USDA and the National Plant Board, no other states made any commitment to conduct a survey for this fly. While the state of Oregon may have some concerns with this pest, they have to prove an absence of it before any action can be taken.
 

Photo Courtesy Ed Show
Photo Courtesy Ed Show

Spotted wing drosophila adults on raspberry.

Posted on Thursday, July 9, 2009 at 9:40 AM

Read more

 
E-mail
 
Webmaster Email: cesacramento@ucdavis.edu